Millipore UFC503024 Regenerated Cellulose Amicon Ultra Centrifugal Filter for Protein Purification and Concentration

The Amicon Ultra-0.5 device is a versatile tool that can be used for various applications, including protein purification, Western blotting, and protein extraction. The device comes with two microcentrifuge tubes, one for collecting the filtrate and the other for recovering the concentrated sample.

The Amicon Ultra-0.5 device is designed for use with a fixed-angle rotor, with a centrifugation speed of 14,000 x g for the concentration spin and 1,000 x g for the reverse spin. The device has a length of 4.99 cm (0.196 in) and is made of regenerated cellulose, a durable and reliable filter material.

The Amicon Ultra-0.5 device is primarily used for protein concentration and desalting, and is classified as a Protein Sample Prep centrifugal filter unit. The device is clear in color and is part of the Ultracel media brand. It has a minimum final concentrate volume of 15 μL and is an ultrafiltration device with a nominal molecular weight limit (NMWL) of 30 kDa.

The Amicon Ultra-0.5 device is designed to concentrate 500 μL of sample down to 15 μL, resulting in a concentration factor of 25x to 30x. The device offers high sample recovery, with greater than 90% of the dilute starting solution being recovered. Additionally, the fast processing time of 10 to 30 minutes ensures efficient sample processing.

One of the key features of the Amicon Ultra-0.5 device is its reverse spin capability, which provides consistent recoveries. This feature is particularly useful for concentrating biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms, as well as for purifying macromolecular components found in tissue culture extracts or cell lysates.

The Amicon Ultra-0.5 device can also be used for a variety of other applications, such as primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC. Furthermore, it can be used for desalting, buffer exchange, protein dialysis, size exclusion, and protein fractionation.

The device is made from a housing of styrene/butadiene and a cap and tube of polypropylene, ensuring a durable and reliable construction. The Amicon Ultra-0.5 device is a versatile and efficient tool for researchers and scientists working in various fields, including biology, biochemistry, and molecular biology.

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