Millipore UFC910024 Amicon Ultra Centrifugal Filter Unit with PLHK Ultracel-100 Membrane, 15mL Capacity, 100kDa NMWL

The Millipore UFC910024 Amicon Ultra Centrifugal Filter is a single-use, 15mL filter that is designed to concentrate, purify, and desalt proteins from dilute and complex sample matrices. It features a vertical membrane design that allows for sample recovery of typically greater than 90% of the dilute starting solution, with a processing time as fast as 10 to 15 minutes. This vertical design also minimizes solute polarization and fouling, enhancing the filter's efficiency.

The filter consists of a filter device, filtrate tube, and cap, and is used in conjunction with a 200μL pipette (sold separately) to collect the concentrated sample from the filter device's sample reservoir, while the filtrate collects in the filtrate tube. Both the filter device and tube have graduations to monitor the sample volume.

The filter utilizes a low-binding Ultracel-100 regenerated cellulose membrane that is heat-sealed to minimize solute loss. This membrane has a nominal molecular weight limit (NMWL) cut off of 100 kilodaltons (kDa), making it suitable for a wide range of protein applications.

The Millipore UFC910024 Amicon Ultra Centrifugal Filter is compatible with centrifuges that have either a swinging-bucket or 35-degree fixed-angle rotor, and can accommodate 50mL tubes. The filter device is made of copolymer styrene-butadiene, the filtrate tube is polypropylene, and the filtrate cap and liner are polyethylene, ensuring compatibility with biological fluids and aqueous solutions.

These filters are sold in packs of 24 and are commonly used in laboratories for protein extraction, purification, and sample preparation. Millipore, the manufacturer, is a leading supplier of life science, filtration, and laboratory supplies, serving the research, food and beverage, and pharmaceutical industries. The company was founded in 1954 and is headquartered in Billerica, MA.

In summary, the Millipore UFC910024 Amicon Ultra Centrifugal Filter is a versatile and efficient tool for concentrating, purifying, and desalting proteins from dilute and complex sample matrices. Its design features, such as the vertical membrane and low-binding membrane, contribute to its high recovery rates and fast processing times, making it a valuable asset in various laboratory applications.

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